TITLE:

METHODS FOR RIGID-BODY AND FLEXIBLE FITTING IN 3-D ELECTRON-MICROSCOPY IMAGES

DATE:

Friday, March 28th, 2008

TIME:

3:30 PM

LOCATION:

GMCS 214

SPEAKER:

Julio Kovacs, Abagyan Laboratory, The Scripps Research Institute

ABSTRACT:

I will describe methods developed in our lab to perform the fitting of given atomic structures of protein molecules into 3-D images obtained by electron microscopy. These images, also called maps, are of limited resolution and thus individual atoms are not visible. Therefore researchers resort to fitting techniques in an attempt to test hypotheses regarding the conformation and arrangement of the molecules within the observed maps. These techniques fall into one of two types: rigid-body or flexible fitting. I will discuss one technique of each type and show examples of applications. The rigid-body fitting method is general and so can be applied in other areas; in contrast, the flexible-fitting one is more specific to the geometry and degrees of freedom of protein molecules.

HOST:

Antonia Palacios

DOWNLOAD: