The ability to isolate and concentrate pathogens (bacteria, virus, etc), biomolecules and any sub-micron particle is critical to many biomedical applications, including diagnosis for cancer and infectious disease (SARS, deadly flu strains, STD, etc). Conventional two-dimensional active microarrays have been used with success for the manipulation of biomolecules including DNA. However they have a major drawback of inability to process relatively â?~large-volumeâ?T samples useful in oncology and infectious disease applications. This research presents an active microarray that exploits electrokinetic (electrophoresis and dielectrophoresis) forces for its hybridization method using 3D carbon electrodes that will enable the large volume manipulation for pathogen detection. Carbon electrodes are fabricated using C-MEMS (Carbon MEMS) technology is an emerging fabrication method that enables low cost fabrication of MEMS devices exploiting fascinating physical,

chemical, mechanical and electrical properties of carbon materials. The chip fabricated using C-MEMS technology is packaged and the efficiency of separation and accumulation of the 3D electrodes on the chip is tested by manipulating negatively charged polycarboxylate 2 micron beads in 50 mM histidine buffer.